Pierre@ D OXI²


  1. Introduction

This OXI² Pre-Wash was developed and tested to remove hardened stains and smells caused by urine on carpets. Mammalian excretions such as blood, faeces, urine etc. consist of a combination of ammonia, bacteria, hormones, and uric acid.

As the urine breaks down, the ammonia concentration increases and sulphur-containing organic chemical substances known as mercaptans are then released.

  1. The chemical structure of Urea

The chemical structure of urea is written as (NH2)2CO. This means that two nitrogen-containing, ammonia-derived molecules are joined to a carbon molecule that has a double bond to an oxygen molecule.

  1. Contents

Dog urine contains urea, creatine, uric acid, carbohydrates, enzymes, fatty acids, hormones, sodium, potassium, chloride, magnesium, calcium and ammonia.

  1. Animal-related marks and stenches

When a dog smells another dog’s urine, it can detect the hormones, such as estrogen or testosterone that is present in the urine. That stimulates their reaction to mark or remark the area.

DO NOT CLEAN such affected areas with products containing ammonia. For urine turns as explained above, into ammonia, react then on a molecular level to the chemical structure, worsening the urine stench as it releases it in a more concentrated form.

For the above reasons, the Pierre @ Dieregesondheid OXI² PRE -WASH stain remover was developed, whereby it uses oxygen to remove and break the particles down.


  • No environmental hazards – breaks down to oxygen in your wash water
  • Colour safe and fabric safe. It brightens colours
  • Continual use will not cause yellowing or greying of cotton fabric
  • Effective stain removal in a broad range of water temperatures
  • Prevent fabrics from becoming yellowed or darkened
  • Does not weaken the strength of fabrics like chlorine bleach
  • In the laundry 02 Pre-Wash is used to de-stain, deodorize, and whiten. It is very effective as a laundry pre-soak for heavily stained articles
  • It is very effective as a laundry pre-soak for heavily stained articles
  • For light soils: add 30ml (30g) of 02 Pre-wash with your laundry detergent per load. For moderate soils: use
  • 60ml (60g) and for heavily soiled articles: use 120g (120ml)
  • For whitening old linens and yellowed window shears, deodorizing and cleaning tennis shoes, or to get rid of yellow armpit stains on shirt, soak in 60ml (60g) of 02 Pre-Wash per 3.80-liter water for at least one hour, or overnight, then rinse.


Pierre @ D OXI² Pre-Wash will remain active for 5-6hrs, after which they should be discarded. Unused material may be poured down the drain. It will help clean and deodorize your disposal or toilet.02 Pre-Wash is most effective when mixed and used in warm to hot water (65degrC)

  • Mix 120ml (120g) of Pre-Wash in 3.8 litres of warm or hot water
  • Heavy Cleaning: Mix 240ml (240g) in 3.8 litres of warm or hot water
  • Soaks: Mix 60 – 120ml (120g) in 3.8 litres of warm or hot water
  • Paste: Mix 30-60ml/g with just enough water to make a paste
  • To de-stain and deodorize carpet, start by mixing a general cleaning solution. Remove as much of the dirt or
  • liquid as possible.
  • Spray Pre-Wash solution on spot, work into area with a brush and let stand 5-10 minutes.
  • Rinse thoroughly by pouring fresh water over spot, blot up with a clean cloth or towel.
  • Vacuum thoroughly when completely dry.
  • Always test for color fastness before applying OXI² Pre-Wash.
  • Carpet cleaning machines add 30ml/g per 3.8litre of warm water in the solution tank.
  • For stained plastic ware, dishes, dish rags and dish towels add 30ml/g to 60ml/g to the container to be cleaned or to the dishwater and soak for at least 15 minutes.
  • To keep your drains and disposals clear and smelling fresh add 30g/ml in 240ml/g of hot water and allow solution to sit in drain overnight
  • To de stain your coffee pot, add 30g/ml if 02 Pre-Wash into a coffee pot of hot water, soak 1015minutes and rinse thoroughly.
  • To eliminate mould, mildew and other organic stains, mix general cleaning solution in a squirt bottle and apply with a soft cloth, let stand 10-20minutes, wipe and rinse thoroughly. Cutting Boards, Tupperware, and Kitchen Counter Tops
  • To clean and deodorize your whole kitchen use a general cleaning solution and apply with a soft cloth, squirt bottle or sprayer, let stand 5-10 minutes and rinse.
  • For extra strong stains, soak item with an extra strong solution for 15-20minutes, rinse thoroughly
  • For septic systems add 120ml/g of Pre-Wash toilet and flush into the septic system twice a week. For toilets add 90g/ml, wait for 15 minutes then scrub.
  • For shower curtains soak in 30g per 3.8 litre of hot water, for mildew stains soak overnight.
  • For bathtub and shower use make a paste with hot water, scrub with cloth or sponge, wait 15 minutes then rinse or use
  • To remove mould and mildew prior to painting or to clean up water damage, mix a soaking solution, apply to surface, and scrub with a brush or sponge. Let stand 20-30 minutes and rinse thoroughly.
  • To remove weathered stains for wood decking, fencing, siding, concrete, and stucco, use a general cleaning solution and apply with a clean lawn sprayer. Let stand 10-30minutes, scrub if necessary and rinse thoroughly.
  • Or mix a general cleaning solution in a bucket and apply with broom or brush, then scrub and rinse thoroughly
  • Use the general cleaning solution and apply to fireplace or bricks with a scrub brush or broom and allow standing 10- 20minutes and rinsing thoroughly with fresh water.
  • Scrub before rinsing if necessary
  1. YARD
  • For deodorizing garbage cans add 15g/ml per 3.8litre of water and let soak. For lawn furniture add 60g/ml to hot water, scrub, wait 15 minutes, then rinse. For all weather carpeting add 120g/ml to 3.8litre of warm water scrub with a stiff broom, wait 20 minutes and rinse.
  • To de-stain and deodorize carpets add 30g/ml Pre-Wash per 3.8litre of carpet shampoo prior to cleaning.
  • For tiles add 180g/ml per 3.8litres of hot water, spray on tiles, wait 15minutes then rinse. For mops add 90g to 3.8litre of hot water, soak overnight then rinse.
  • For mildew removal or to clean basement floors after water damage from flooding add 120g/ml to 3.8litre of hot water, scrub surface, wait 30 minutes then rinse.
  • To clean and deodorize cat litter boxes soak then in 902g/ml if Prewash per 3.8litre of water for 20 minutes then rinse.
  • For urine, faeces or vomit stains on carpets or upholstery add one teaspoon of 02 Pre-wash to 240ml water, wipe onto stain, wait 10 minutes the rinse
  • To clean and deodorize coolers add 60g/ml if Prewash per 3.8 litres of hot water soak for 20 minutes, then rinse, for boat sails, cushions, ropes, camping gear, tent sets add120g/ml per 3.8 litres of hot water soak for 30 minutes then rinse

Pierre van Niekerk ©2


Pierre@ D: Bio Enzymes

Natural Biological Products in Agricultural Waste Treatment


There is a sincere need and demand for safe and natural products to solve and deal with stenches and animal waste in animal shelters, chicken houses, piggeries, cattle kraals, slaughter houses and dairies. In household’s urine stenches or often a problem, where children are involved and general smell and hygiene is important.
Many of the shelve and household chemicals are harmful. Not only to the environment, but to man and animal in general causing skin problems, asthmatic conditions and allergies. Typical non-specific household cleaning products are harsh and contains harmful corrosive substances. Not safe and in effect dangerous to children and to pets.

When spilled, harsh chemicals can destroy the balance of the natural biotic environment, as well as good bacteria and insects of value to our surroundings and environment.

Thus, the need and quest for effective, biological safe and natural products is the quest for most informed people. Three words should be associated with Biological products.

The product must be safe, pure and effective.
Per Biological definition it would mean the following;
1. Safety
Relatively free from harmful effects and or substances, direct or indirect, when a product is sensibly administered.
2. Pure
Purity means relative freedom from unimportant matter in the finished product. Pure and purity means free from residue, fillers or moisture and or unstable substances.

3. Effective
Effective refers to the specific ability or capacity of the biological product, and best as indicated by appropriate laboratory tests, to explain and produce a given result.
Natural bacteria as a Bio degradation agent.

Bio-Enzymes are a biological formulation of multi-purpose bacteria, formulated to have a multi- purpose and broad range of natural degradation abilities and functions. In their natural environment, bacteria produce hundreds of enzymes in response to the organics present in their environment.

This group of Bio-Enzymes produce extracellular enzymes with the intention to break down proteins, starches, fats, oils, greases and toilet tissue into smaller particles, outside the bacterial cell. The bacteria then transport the smaller particles across their cell membrane for use as an energy source and for generating new cellular components.

Why is this an efficient method in stench and waste control?
Since this group of selected bacteria detect organics material as a potential food source, they produce specific enzymes to breakdown the organics present. In areas as mentioned with urine and organic waste, this selected consortium of bacteria will produce key extracellular enzymes including amylase, cellulase, lipase and protease.

This Biological method is a focused, complete and very efficient natural system, leaving no harmful waste to the environment. Many different enzymes are required to break a substrate (Urine, faeces, blood, etc.,) completely.

Extreme functionality
This group of Bio Enzymes can be therefore successfully used for the maintenance and functional working order of household drain lines, slaughter houses, French drains, grease traps, improving septic tanks, nutrient rich waste environments such as dairies, butcheries.

Although many bacteria can utilize these organics as food sources, it is this group of Bacillus bacteria with the rapid production of key enzymes that provide the most dramatic effects in waste degradation, odour control in piggeries, pens, kraals, shelters, toilets, latrines and slaughter houses. Extremely safe and effective in with in-house stenches and odours and stenches caused by pets.

Safety of Pierre@ D Bio-Enzymes
Pierre@ D contains a blend of safe Bacillus microorganisms. Toxicity studies done by an independent laboratory shows that Pierre @ D Bio-Enzyme consortium of microorganisms has no acute oral toxicity, no acute dermal toxicity, and no acute inhalation toxicity at maximal test dose.

Acute dermal irritation and acute eye irritation studies classify Pierre@ D Bio-Enzymes as non-irritating it does not elicit a skin sensitization reaction.

Bio Enzyme Product Data Sheet

1. Drain lines and grease traps – degrades and eliminates organics found in drain lines and grease traps. Regular addition of PIERRE @ D BIO ENZYME maintains a cleaner and odour-free system.

2. Septic and waste treatment – maintains effective activity in septic systems, eliminating the need for excessive pumping.

3. Eliminates odours caused by incomplete digestion of malodorous volatile fatty acids.

4. Bathroom cleaning and odour control: penetrates cracks, crevices and pores of surfaces where organics accumulate, removing the organics leaving a visually cleaner surface.

5. Provides long term odour control by removing the organics that cause odours and prevents their return.

1. A stable consortium of safe Bacillus spores
2. Production of multiple enzymes providing a wide range of degradation capabilities
3. A synergistic blend that works in concert to provide superior performance across multiple applications
4. Excretion of high levels of amylase, cellulase, lipase and protease enzymes
5. Ability to work under aerobic and anaerobic conditions
6. Single product simplicity for multi-application flexibility

Product Characteristics
1. Bacteria Counts: 5.4 X 107 cfu/ml
2. Bacteria Type: Blend of Bacillus Spores
3. Salmonella: Not detected
4. Appearance: Creamy white
5. Fragrance: Pleasantly perfumed
6. Shelf-life: One year; maximum loss of 1.0 log at recommended storage conditions

Dose Rates: (Follow dilution rate)
PIERRE @ D BIO ENZYME can be diluted to the following ratio;

1. 1:9 (One-part BE (BIO ENZYME) and 9 parts of water. The blend must be used on the day and any reaming liquid should be discarded.

Reduction of high solids and crusting of waste: liquefaction and cleaning of cowsheds, piggeries, poultry farms etc.) Area Initial Dose Rate Regular Maintenance Rate Method of


General aid to the processing process.
Area Initial Dose Rate Regular Maintenance Rate Method of Application
Trickling filters

Anaerobic digesters Retention Ponds Activated sludge 1 kg/ 4.5 million liter’s

500g/ 45 000 liter’s 500g/4.5 million liters per week
Repeat for 3 days then per week Add to primary settling tank
Add to inflow pipe

For easier handling of high protein/fats in concentrated areas.

Reducing blockage of drains, pipes: treatment of effluent not on main drainage: reduction of odours.


Is designed as a bio-technical aid to treatment of organic waste material offering the following advantages:
1. Liquefaction & reduction of solids
2. Reduction of odour
3. Easier disposal of waste
4. Aids general cleaning of soiled areas
5. The safety in operation of effluent systems offers a viable alternative to current processing techniques using a bio-technical approach.

How does PIERRE @ D BIO ENZYME Liquid out-perform various harmful pathogens?

The SABS Standard requires a 5-minute killing time for the most common hygiene microorganisms such as S. aureus, E. coli and P. aeruginosa.

From a hygiene point of view, especially where pets and farm animal’s health or zoonotic diseases are at stake, hygiene factors are of great importance and a critical management matter. Such is products used in creating a hygienic environment.

The responsible value of a healthy and hygienic environment would be to sanitize the working environment effectively before continue working. It could be food preparation, preparing feeding for dairy calves, chicken houses, bird cages, shelters, restaurants, home cooking and food preparation.

The value could also be the sanitizing of public areas, where hygiene factors such as toilets or urinals or cleaning hard surfaces in shops the public use. The same principles and values and codes reign in households, public schools, offices and other public areas.

Mode of Function of Bacillus bacteria as Bio-Enzymes
In contrast to the SABS standard, being the quickest time a sanitizer (QAC- based) is supposed to kill, Bacillus-containing sanitizers such as the Pierre @ D Bio-Enzymes operate in a different mode. Bio-Enzymes are live bacteria that needs to compete with other microorganisms for a food sources. Hence the longer contact time required by these bacteria to kill its competitors.

1. The pattern that bacillus bacteria uses, (as seen in the study), is to kill or out compete the other bacteria. The answer lies in its morphological structure. Where Bacillus bacteria grows, other microorganisms will move away from that specific waste food source.

2. Bacillus bacteria have a larger morphological structure than other organisms. It creates a zone of inhibition, preventing growth of other microorganisms (known as morphological inhibition).

3. This ensures fewer numbers of other microorganisms surviving, but a rapid growth of bacillus bacteria absorbing waste product such as sewerage, milk spills, waste pills, grease traps etc.

Important knowledge to cfu Calculation
For Bio Enzymes to be most effective and efficient, it is always important to know the cfu value of any biological product that has been diluted. Or in its concentrated form. This is to know if the product you have diluted has sufficient active ingredient to fulfil the desired outcome in waste or stench management:

The ready to use (diluted) product PIERRE @ D BIO ENZYME following thee analyses within SAP – shelf life is typically one year. The cfu count on the diluted product is calculated on a mass balance as follows:

1. Let a = Volume of Bio Enzyme
2. And the concentration = 10 X FF
3. Let a = 1 gallon
4. Let b = volume of water = 9 gallons
5. Let c = volume of Bio Enzyme
6. And a + b = c
7. c = 10 gallons

Doing a mass balance on the spore count:
1. There are 2 x 1012 cfu in 1-gallon Bio Enzyme 10 XFF
2. Count of spores in c = unknown = z Then 2 x 1012a + 0b = zc
3. Then [(2 x 1012 x 1)/10] = [(z x 10)/10]
4. 2 x 1011 cfu/gallon of PIERRE @ D BIO ENZYME

Converting from gallons to liters
2 x 1011 spores 1 gallon
1 gallon 3.8 liters

= 5.2 x 1010 cfu/liter of PIERRE @ D BIO ENZYME
Or 52 billion CFU/liter

The information contained in this leaflet is to the best of our knowledge, true & accurate, but any recommendations or suggestions which may be made are without guarantee since the conditions of use are beyond our control. No license or immunity under any patents is granted or implied.

Myte in die ore


Oormyte of gis in die ore.


Eksterne oor infeksie (Otitis) is ‘n infeksie van die oorkanaal.  Diere se oorkanale is sensitiewe dele van hul oorkanaal en word maklik deur ongewenste organismes geïnfekteer.  Die Literatuur is van mening dat 80% van diere wat sukkel met oorprobleme die diere is met lang ore, oormatige groei van hare in die ore, diere met ‘n gebrek aan lugwegvloei wat dit vogtig hou en ‘n omgewing skep wat die voortbestaan van bakterie-groei en myte bevorder.



Verskeie faktore dra by tot die ontwikkeling van oorkanaalprobleme.  Die Chinese Sharpei is veral vatbaar vir oorkanaalprobleme weens hul nou oorkanale wat tot gevolg het dat bitter min lugvloei in die kanaal plaasvind om dit droog te hou en dus vir bakterieë en ander organismes die ideale omgewing skep.


Tekens van oorinfeksies en oormyte

  • Skud van kop
  • Krap van ore met pote
  • Vryf van ore
  • Ander honde wat daarin lek en/of ruik
  • Die kop word gewoonlik laag gedra
  • Oorflappe wat stukkend is, haarloos is en opgewel is
  • Huilgeluide wanneer daar aan die oor geraak word
  • Olierige uitskei vanuit die oor
  • Doofheid
  • Vrees vir klank
  • Reuke vanuit die oor

Tipe van oorinfeksies (terme in Engels sodat lede dit self kan naslaan op Google)

Meer as een tipe bakterie kan by oorinfeksies betrokke wees, wat die behandeling daarvan met antibiotika bemoeilik.

  1. Ceruminous otitis” gaan gewoonlik gepaard met primëre “seborrhea”. Dit is ‘n oormatige opbou van geel, olierige was in die oorkanale wat die ideale medium is vir bakterieë en gis om op te bou.  Behandeling is gerig om die ore en oorkanaal skoon te hou en ook die ander velkondisies te beheer.
  2. Bacterial otitis” in sy akute vorm word gewoonlik veroorsaak deur die bekende “Staphylococci” bakterie. Die uitskeiding is natterig en gewoonlik ligbruin.  Kroniese infeksies word veroorsaak deur die “Proteus” en/of “Pseudomonas” bakterie, wat se afskeidings kenmerkend geel of groenerig is, alhoewel daar al uitsonderings was op hierdie reël.
  3. Gis infeksies (Yeast or fungal infections”) kan die gevolge wees van antibiotiese behandeling vir bakteriese oorinfeksies. Kan ook die gevolg van honde wat sukkel met atopiese dermatitus of “atopic dermatitis”, voedsel veroorsakende dermatitis, en “seborrheic” velkondisies. Kenmerkend van gis is ‘n bruin, olierige was uitskeiding met ‘n kenmerkende stank en ore wat inflammasie het en vogtigheid in die ore.  Hierdie kondisies bly voortbestaan totdat onderliggende velsiektes opgelos is.
  4. Proteien intolleransies – Sien skrywe hieroor.




Pierre van Niekerk ©

Uriensamestelling van honde katte. Skoonmaakmiddels

Pierre @ Dieregesondheid Skoonmaakmiddels:  


Soos genoem, is die reuk van troeteldiere se uriene in die huis ‘n uitdaging waarmee elke diereliefhebber mee uitgedaag word. Hoekom ruik (stink) dit so en wat kan mens daaromtrent doen?

Waaruit bestaan uriene

Die elemente in uriene wissel matig soos wat die dieet wissel, die tyd van die dag speel ‘n rol en ook wateropname. Dit bly egter redelik konstant in sy elemente en samestelling.

Uriene is ‘n kombinasie van water, ammoniak, bakterie, hormone, kreatien, potassium, soute, magnesium, kalsium, vetsure en ensieme.

Dit is alles natuurlike organiese produkte wat deur die natuurlike prosesse van die niere, blaas, urienwegstelsel, geslagsorgane verwerk en uitgeskei word. By manlike diere speel die prostaat n rol en by vroulike diere die afskeiding van hul normale siklusse.

Wat veroorsaak die skerp reuk

Soos wat die water uit die uitgeskeide uriene verdamp, verhoog die ammoniak konsentrasie van die urien.  Daarmee saam ontsnap die waterstof molekule en verbind dit met ‘n swaelmolekule. Op sy beurt vorm dit “Thiole “of “Mercapans”

Thiole is die chemiese elemente wat gevind word by vars gesnyde uie of die elemente in ‘n muishond se uriene wat die skerp stank afgee. Thiole is ook te vinde in natuurlike gas en petroleum en die verrotting in produkte soos kool.

Waarmee word dit skoongemaak

Bogenoemde is presies die redes waarom honde en katte se uriene nie met ammoniak tipe produkte skoon te maak nie. Jy voeg net meer ammoniak tot die probleem toe. Buitendien, ammoniak is ‘n natuurlike element in honde se uriene en kan juis daarom dink dit is natuurlik om wel hier te urineer, want sy ouers doen dit dan gereeld.

Dit is daarom beter om glad nie met ammoniakformulasies in skoonmaakmiddels te werk  waar troeteldiere in die huis of hokke is nie. Dit stimuleer hul om daar te urineer.

Dit verduidelik waarom ander skoonmaakelemente in die Pierre@ D Skoonmaakmiddels produkte gevind moes word om die probleem wat uriene meebring, beter en tog veilig te bestuur.

Chemiese samestelling

In die Chemie sou uriene so geformuleer word. (NH2) 2CO.

Hoe beïnvloed dit die gedrag van troeteldiere

Veel meer kan geskrywe word rakende die uriene van mens en dier omdat beide soogdiere is. Wat beteken dat die uriensamestelling maar baie dieselfde is.

Weens hul vermoë om miljoene kere beter as ‘n mens te ruik, speel die mens se persoonlike higiëne ‘n baie groot rol in die gedrag en optrede van diere se urinering op matte in huise.  Dit is gevul met ammoniak, stimulerende hormone en ander chemiese stowwe wat hul breine stimuleer en reaksies ontlok.

Die afskeiding (stank) van stofmyte, goggas en ander organismes wat almal reuke (thiole) in beskerming, voortplanting en vlug of veg, beïnvloed jou hond se gedrag t.o.v. urinering in die huis.

Bogenoemde faktore was in berekening gebring tot die

  1. Pierre@ D Urien-neutraliseerder in poeiervorm
  2. Die Pierre@ D lugverfrisser
  3. Die Pierre@ D Multi-aktief
  4. Die Pierre@ D matsjampoe en
  5. Die Pierre @ D Oxy² Bleach ontwikkel is.

Pierre van Niekerk ©