Pierre @ Eco-Health: Poultry Litter Bio-Enzyme

Chemical-free non corrosive poultry litter treatment for incredible ammonia, pathogen and odour control. Increases poultry health by forming a continuously working protective biological barrier.

Substance Challenges in Chicken Houses

Ammonia is a serious and continued challenge for poultry and the farmer. It’s corrosive to equipment and damaging to the environment. Conventional wisdom is to change the acidity of the litter to limit ammonia.


Ammonia is a volatile compound and above 7.2 PH it acts as a gas with adverse effects. Below 7.2 PH it’s converted into ammonium.

This process fluctuates continually, and as ammonia levels increase costly ventilation must be used, which is not a carbon green solution. Harsh and expensive corrosive chemicals are needed to increase PH in an increasing and difficult cycle.


Mode of Action:

Eco-Health Poultry Litter Bio-Enzyme takes a novel approach to ammonia by involving:

  • Biological Conversion Approach:

A proprietary microbial consortium targets ammonia and permanently converts it to ammonium nitrate fertilizer.

  • Lignin / Carbon Approach:

Ammonia gas levels increase when there is a lack of carbon in the litter bed. When the amount of carbon is less than that required for converting available nitrogen into protein

Eco-Health Poultry Litter Bio-Enzyme makes better use of the available carbon to eliminate the excess nitrogen as ammonia. It adds considerable carbon in the form of lignin, a crucial factor in reducing ammonia.

  • Organic Acid Ammonia Chelation Approach:

Eco-Health Poultry Litter Bio-Enzyme helps to reduce ammonia through a process called chelation.


Competitive Exclusion:

Health Challenge:

  • Pathogens and their detrimental effects in the poultry environment are well documented.
  • They are extremely dangerous, and very difficult as well as costly to control.
  • They naturally develop resistance and immunity to substances that harm or kill them, in other words harsh chemicals, disinfectants, sanitizers and antibiotics.
  • They build up immunity and resistance over time and become harder to kill the next time.
  • In this vicious unstoppable cycle extra harsh chemicals and more powerful and costly disinfectants are always needed.


Eco-Health Poultry Litter Bio-Enzyme Solution:  

  • Eco-Health Poultry Litter Bio-Enzymes are safe and good probiotics which naturally occur in nature and are not genetically modified in any way.
  • In every few drops of Eco-Health Poultry Litter Bio-Enzyme product, there are tens of millions to billions of good bacteria.
  • When they are applied, they quickly go in search of food and space, and rapidly outcompete the bad bacteria for their food source.
  • Eco-Health Poultry Litter Bio-Enzyme rapidly keep on reproducing at an exponential rate and completely colonize the area or system.
  • Ensures in a short period of time that the bad pathogens in the litter starve off and die.
  • An incredible fact is that where conventional disinfectants usually stop working after application, the Eco-Health Poultry Litter Bio-Enzyme process can continue for hours, days and more after application, so the effect of a healthy flock environment is long and lasting.


Eco-Health Poultry Litter Bio-Enzyme is Non-Corrosive:

  • Corrosion of expensive equipment is a serious problem that ruins the profit of poultry operations.
  • Corrosion can happen anywhere in a poultry operation, and current practices in broiler operations only increase the opportunity for corrosion.
  • Corrosion is simply the deterioration of materials through a chemical reaction with the environmental condition.
  • Perhaps the most noted corrosion occurs with the rusting of iron or other such metals.
  • Corrosion is not confined to just metal as it affects plastics, concrete, wood and other materials.
  • The single most deconstructive chemical created by a poultry operation is ammonia.

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  • Ammonia will always be produced in a poultry house as it is a reaction between manure, moisture, and microbiology.
  • Typically, the higher the humidity of a poultry house the greater ammonia levels become.
  • Ammonia is well noted for its detriments to the welfare of the birds, but we rarely hear about this deterioration of metal components in a poultry house.
  • This deterioration includes valuable fasteners, heaters, walls, ceilings and any other metal object.

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  • Many poultry farmers combat ammonia by applying an acid product for poultry litter treatment.
  • The purpose of acid products is to lower the Ph value of the litter.
  • The ends of the spectrum on the Ph scale are ammonia and acid. A very rudimentary thought would be “Where there is ammonia let’s apply acid to make it neutral”
  • This solution has not been thought out. It would take as much acid as ammonia to make a fair play and truly offset the ammonia.
  • That would be extremely expensive not to mention dangerous.
  • The use of such products leads to elevated acid levels that in turn create an even more corrosive poultry house environment.
  • Products that convert into acid materials lead to groundwater contamination.

normal-chicken-poopEco-Health: Poultry Litter Bio-Enzyme is completely safe and is not corrosive.

It’s made up of entirely organic constituents and poses no problem when contacted with skin or animals. By implementing a complete poultry management system with Eco-Health Poultry Litter Bio-Enzyme, a farmer is extending the life of his equipment among other things and is improving his return on investment.

Long Term Ammonia Control:

  • For long term ammonia control, conventional wisdom has been to spread powerful acid products into the poultry house.
  • The logic is that by making the pH level of the litter low enough, if it’s more acidic, then the ammonium can’t in turn convert into ammonia gas.
  • The faulty rationale in this instance is that the ammonium is still there available.
  • The acid doesn’t do away with it. It just traps the ammonium and delays the inevitable problem.
  • Eventually, the poultry manure itself will raise the pH, and the ammonia problem will return.

Eco-Health Poultry Litter Bio-Enzyme works completely differently to acid treatments.

Eco-Health Poultry Litter Bio-Enzyme beneficial bacteria give the natural process a head starts which break the ammonium down faster than it normally would.

  • A specific strain of bacteria is present to bind the ammonium with nitrogen to make ammonium nitrate which is conventional plant fertilizer.
  • Once bound up as fertilizer, it can’t change back, and ammonia is permanently eliminated.
  • With Eco-Health Poultry Litter Bio-Enzyme, competitive exclusion takes time for a colony to establish, and after a few days’ ammonia levels begin to plummet and one will continue to see low ammonia levels.
  • When dealing with a poultry house that has been previously treated with Eco-Health Poultry Litter Bio-Enzyme ammonia levels will be much lower than a house treated with acid.
  • Adding a fresh application Eco-Health Poultry Litter Bio-Enzyme drastically improves the situation.
  • With repeated use of Eco-Health Poultry Litter Bio-Enzyme ammonia problems are nearly eliminated.

Easy Liquid Application:

Eco-Health: Poultry Litter Bio-Enzyme is an easy to apply liquid treatment.

  • Simply mix our highly concentrated formula with water in a clean pressure sprayer and apply thoroughly on the poultry house floor.
  • Conventional dry bulk products are difficult to handle and are labour intensive. In the time what typically takes a few workers to cover a brood section of a broiler house, an entire house can be covered by one person with Eco-Health Poultry Litter Bio-Enzyme.
  • There is also no need for P.P.E.’s (Personal Protective Equipment).

Application Instructions:

Step 1: Seal the house after the flock is removed to maintain a higher temperature.

Microorganisms are more effective in warmer temperatures. Ventilate before re-entry for service, or if moisture begins to condensate in the house.

Step 2: Immediately after de-caking, and before adding new bedding, apply Eco-Health Poultry Litter Bio-Enzyme at the rate of 1L per 400 square meters. For each 1L of concentrate dilute with 100L of water. After diluting wait 30 minutes before applying.

  • A clean pressure sprayer which has not been previously contaminated by disinfectants must be used. Evenly cover the entire surface of the litter bed with spray.
  • Ideally seal the house for 24 hours after application. Follow the standard ventilation program before and after bird placement.

Product Features:

  • Non-corrosive
  • Keeps litter dry
  • Reduce mortality rates
  • Reduces odour
  • Maintain body heat
  • Reduce litter bugs
  • Reduce condemnation rate
  • Increase feed conversion
  • Increase flock weight
  • Binds ammonia
  • Produces clean air
  • Lowers utility costs
  • Lowers exhaust fan costs
  • Reduces caking in litter
  • Non-toxic
  • Convenient liquid form
  • Easy to apply liquid
  • Less shavings required
  • Applied with conventional spray equipment

Packaging and Storage:

Packaged in 25L drums. Store in a cool, dry place. Shelf life valid for 1 year.

Gewigsverlies 2: Leptienweerstandigheid en Glycyrrhiza glabra of “Liquorice root.”


Die skrywe beskryf die eerste van enkele kruie wat bepaalde funksies moet verrig in die reeks vetverliesprodukte. Dit skrywe moet saam met ander artikels oor produkte gelees word wat die afgelope tyd geplaas is oor gewigsverlies.


Om gewig te verloor is vir sommige ‘n bitter swaar lewenservaring en in wese ‘n erge stryd en lyding. ‘n Saak van gebed en hoop en ‘n saak waar vele hul eie selfbeeld skend. En “hoop” word ʼn dief van die nag. Een wat nooit waar word nie. ‘n Vet dief van geluk en  ‘n nalatenskap van seer menswees.


Waarskynlik is mense wat gewig wil verloor se pad soveel moeiliker. Moeilker omdat hul nie goeie kennis dra van die faktore wat gewigstoename veroorsaak nie. Maar dan, dan is ook soveel “guru’s” al die antwoorde en slim bemarking, met al die antwoorde en al die planne en pille dat dit mense nog eens moedeloos laat.

Veral as niks werklik gebeur nie.

Leptien, Ghrelien en Kortisol

  • Skrywe 1 beskryf die funksie van die hormoon Ghrelien.
  • Skrywe 2 handel oor die invloed van die hormoon Leptien en Leptienweerstandigheid.
  • ‘n Derde kritiese rolspeler en ook ‘n hormoon, wat deur die byniere afgeskei word, naamlik “Kortisol” word ineengevleg met die skrywes omdat dit n belangrike rol speel by gewigstoename.

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Leptien Kode

Die liggaam is Biologies geprogrammeer om energie (vet) te preserveer.

Die hormoon wat jou brein opdrag moet gee om van gestoorde energie gebruik te maak, is Leptien. Dit moet die brein deur die bloedstroom van die regte inligting voorsien.nlig. Sou daar genoeg gestoorde energie beskikbaar wees, moet die brein in normale omstanbdighede dit eersten verbruik en nie opdragte uitstuur om energie te stoor nie.

Wanneer die brein nie die seine in die bloedstroom reg lees, kan ontvang of dit reg te intrepreteer nie, ontstaan ‘n toestand van Leptienweerstandigheid.

Leptienweerstandigheid is die een faktor wat in enige gehalte gewigsverliesprogram aangespreek word. Die program moet daarin slaag dat die brein deur die bloedstroom die regte seine en kodes ontvang.

  • Om gestoorde energie eerste te gebruik.
  • Om nie te dink dat die liggaam in ‘n stresmode is en als wil stoor nie.

Kry ‘n gewigsverliesprogram die ideaal reg

Sal vetverlies vir niemand ‘n probleem wees nie. Leptienweerstandigheid word in die Literatuur aangespreek met die wortel van ‘n plant in die volksmond genaamd Glycyrrhiza glabra of “Liquorice root.”

  1. Glycyrrhiza glabra of “Liquorice root.”

Dit is die wetenskaplike naam vir ‘n flavonoïed ekstrak wat in die Literatuur bewys is dat dit die brein se leptienreseptore stimuleer,  sodat jou brein nie sonder inligting funksioneer en dink die liggaam is in oorlewing nie.



  • Liquorice wortel is sowat 50 keer soeter as suiker. Ander bronne beweer meer.
  • Hierdie produk skakel die brein se leptienreseptore aan sodat dit die regte boodskappe aan die brein, weer in die bloedstroom gegee kan word en, dat dit raakgesien kan word.
  • Glycyrrhiza glabra vervul ook ‘n ander belangrike funksie.

Dit stimuleer ook die PPAR-alpha proteïen, wat in die Literatuur beskrywe word as die proteïen wat witvet of “visceral fat” afbreek.

  • PPAR-alpha is daardie groep proteïene

Wat die Literatuur beskrywe as ‘n proteien wat;

  • Die afbreek van vet stimuleer
  • Die metabolisme reguleer
  • Bloedsuikervlakke af te bring
  • Stysels, vette en proteïene af te breek
  • Konsentrasievlakke te stabiliseer
  • Gemoedstoestande stabiliseer en
  • Verminder jou insulienweerstandigheidsvlakke , wat in effek beteken jou liggaam kan weer sensitief word vir insulien.


Glycyrrhiza glabra help dus,

  • deur die stimulering van die PPAR-alpha proteïne, wat fokus die wit sagte maagvette of “visceral fat.”
  • Dit is die vette wat om die maag en organe versamel en bekend is vir hul bydrae tot hartvatsiektes, kanker, depressie en vele ander probleme.

Glycyrrhiza glabra help dus,

  • Met minder eet omdat dit ‘n natuurlike versadiging meebring.
  • Glycyrrhiza glabra verminder eetlus natuurlik deur die leptienreseptore se kommunikasie aan te spreek en te sorg dat die boodskap korrek oorgedra word.
  • Nog ‘n belangrike “maer maak” ensiem word deur die brein gestimuleer, nl., 

Adenosine 5′ monophosphate-activated protein kinase” of AMPK .

Dit is ‘n ensiem wat bekend staan as die liggaam se “Meester reguleerder van Energie Metabolisasie.” of “Master regulator of energy metabolism.

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Hoekom is dit belangrik om die gedeelte van die brein te verstaan?

Wanneer die “kontrole sentrum van jou brein,” die hipotalamus” wat die outonome senustelsel en die pituïtêre klier reguleer, agterkom dat die liggaam se energievlakke laag is, stimuleer die brein die vorming van AMPK.


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AMPK funksioneer as ‘n “Energie- sensor” binne die selle van die liggaam.

  • Navorsing wil glo dat soos wat die mens ouer word, verminder hul “AMPK aktiwiteite.”  Wat verklaar waarom daar veranderinge in gewig, aptyt en energievlakke plaasvind.
  • Maar vir AMPK is daar ook ‘n natuurlike antwoord. Dit word gestimuleer deur bietjie verhongering en ook vas. Tekorte aan energie stimuleer ook die vorming van AMPK en
  • Adiponektien word dan afgeskei by vas en verhongering. Verhongering kommunikeer deur die bloed aan die brein dat daar te lae energievlakke vir die liggaam bestaan.
  • Wat tot die aktivering van die Adenosien 5′ mono-fosfaat geaktiveerde proteïen kinese” lei.  

Wat inhibeer die funksies en vorming van AMPK

  • Ooreet of dan te veel en aanhoudend eet
  • Verhoogde suiker en styselinames
  • Verhoogde insulienvlakke
  • Hoë bloedsuikervlakke
  • Te veel alkohol
  • Mineraaltekorte onderdruk die nodige vorming van AMPK.

Die ideaal…

Dat ideaal van die Pierre@ Ecohealth program is logies wees. Dit moet op ‘n natuurlike wyse die vorming van AMPK in jou spiere, lewer en selle stimuleer.  Word dit reggekry, versnel dit die metabolisme natuurlik en, dit beteken;

Die aktiewe benutting of verbranding van vet.



Daarom die keuse van Glycyrrhiza glabra. Dit veroorsaak daar kinese van “AMPK” of “Adenosien 5′ mono-fosfaat geaktiveerde proteïen” plaasvind.


Pierre@ D: Bio Enzymes

Natural Biological Products in Agricultural Waste Treatment


There is a sincere need and demand for safe and natural products to solve and deal with stenches and animal waste in animal shelters, chicken houses, piggeries, cattle kraals, slaughter houses and dairies. In household’s urine stenches or often a problem, where children are involved and general smell and hygiene is important.
Many of the shelve and household chemicals are harmful. Not only to the environment, but to man and animal in general causing skin problems, asthmatic conditions and allergies. Typical non-specific household cleaning products are harsh and contains harmful corrosive substances. Not safe and in effect dangerous to children and to pets.

When spilled, harsh chemicals can destroy the balance of the natural biotic environment, as well as good bacteria and insects of value to our surroundings and environment.

Thus, the need and quest for effective, biological safe and natural products is the quest for most informed people. Three words should be associated with Biological products.

The product must be safe, pure and effective.
Per Biological definition it would mean the following;
1. Safety
Relatively free from harmful effects and or substances, direct or indirect, when a product is sensibly administered.
2. Pure
Purity means relative freedom from unimportant matter in the finished product. Pure and purity means free from residue, fillers or moisture and or unstable substances.

3. Effective
Effective refers to the specific ability or capacity of the biological product, and best as indicated by appropriate laboratory tests, to explain and produce a given result.
Natural bacteria as a Bio degradation agent.

Bio-Enzymes are a biological formulation of multi-purpose bacteria, formulated to have a multi- purpose and broad range of natural degradation abilities and functions. In their natural environment, bacteria produce hundreds of enzymes in response to the organics present in their environment.

This group of Bio-Enzymes produce extracellular enzymes with the intention to break down proteins, starches, fats, oils, greases and toilet tissue into smaller particles, outside the bacterial cell. The bacteria then transport the smaller particles across their cell membrane for use as an energy source and for generating new cellular components.

Why is this an efficient method in stench and waste control?
Since this group of selected bacteria detect organics material as a potential food source, they produce specific enzymes to breakdown the organics present. In areas as mentioned with urine and organic waste, this selected consortium of bacteria will produce key extracellular enzymes including amylase, cellulase, lipase and protease.

This Biological method is a focused, complete and very efficient natural system, leaving no harmful waste to the environment. Many different enzymes are required to break a substrate (Urine, faeces, blood, etc.,) completely.

Extreme functionality
This group of Bio Enzymes can be therefore successfully used for the maintenance and functional working order of household drain lines, slaughter houses, French drains, grease traps, improving septic tanks, nutrient rich waste environments such as dairies, butcheries.

Although many bacteria can utilize these organics as food sources, it is this group of Bacillus bacteria with the rapid production of key enzymes that provide the most dramatic effects in waste degradation, odour control in piggeries, pens, kraals, shelters, toilets, latrines and slaughter houses. Extremely safe and effective in with in-house stenches and odours and stenches caused by pets.

Safety of Pierre@ D Bio-Enzymes
Pierre@ D contains a blend of safe Bacillus microorganisms. Toxicity studies done by an independent laboratory shows that Pierre @ D Bio-Enzyme consortium of microorganisms has no acute oral toxicity, no acute dermal toxicity, and no acute inhalation toxicity at maximal test dose.

Acute dermal irritation and acute eye irritation studies classify Pierre@ D Bio-Enzymes as non-irritating it does not elicit a skin sensitization reaction.

Bio Enzyme Product Data Sheet

1. Drain lines and grease traps – degrades and eliminates organics found in drain lines and grease traps. Regular addition of PIERRE @ D BIO ENZYME maintains a cleaner and odour-free system.

2. Septic and waste treatment – maintains effective activity in septic systems, eliminating the need for excessive pumping.

3. Eliminates odours caused by incomplete digestion of malodorous volatile fatty acids.

4. Bathroom cleaning and odour control: penetrates cracks, crevices and pores of surfaces where organics accumulate, removing the organics leaving a visually cleaner surface.

5. Provides long term odour control by removing the organics that cause odours and prevents their return.

1. A stable consortium of safe Bacillus spores
2. Production of multiple enzymes providing a wide range of degradation capabilities
3. A synergistic blend that works in concert to provide superior performance across multiple applications
4. Excretion of high levels of amylase, cellulase, lipase and protease enzymes
5. Ability to work under aerobic and anaerobic conditions
6. Single product simplicity for multi-application flexibility

Product Characteristics
1. Bacteria Counts: 5.4 X 107 cfu/ml
2. Bacteria Type: Blend of Bacillus Spores
3. Salmonella: Not detected
4. Appearance: Creamy white
5. Fragrance: Pleasantly perfumed
6. Shelf-life: One year; maximum loss of 1.0 log at recommended storage conditions

Dose Rates: (Follow dilution rate)
PIERRE @ D BIO ENZYME can be diluted to the following ratio;

1. 1:9 (One-part BE (BIO ENZYME) and 9 parts of water. The blend must be used on the day and any reaming liquid should be discarded.

Reduction of high solids and crusting of waste: liquefaction and cleaning of cowsheds, piggeries, poultry farms etc.) Area Initial Dose Rate Regular Maintenance Rate Method of


General aid to the processing process.
Area Initial Dose Rate Regular Maintenance Rate Method of Application
Trickling filters

Anaerobic digesters Retention Ponds Activated sludge 1 kg/ 4.5 million liter’s

500g/ 45 000 liter’s 500g/4.5 million liters per week
Repeat for 3 days then per week Add to primary settling tank
Add to inflow pipe

For easier handling of high protein/fats in concentrated areas.

Reducing blockage of drains, pipes: treatment of effluent not on main drainage: reduction of odours.


Is designed as a bio-technical aid to treatment of organic waste material offering the following advantages:
1. Liquefaction & reduction of solids
2. Reduction of odour
3. Easier disposal of waste
4. Aids general cleaning of soiled areas
5. The safety in operation of effluent systems offers a viable alternative to current processing techniques using a bio-technical approach.

How does PIERRE @ D BIO ENZYME Liquid out-perform various harmful pathogens?

The SABS Standard requires a 5-minute killing time for the most common hygiene microorganisms such as S. aureus, E. coli and P. aeruginosa.

From a hygiene point of view, especially where pets and farm animal’s health or zoonotic diseases are at stake, hygiene factors are of great importance and a critical management matter. Such is products used in creating a hygienic environment.

The responsible value of a healthy and hygienic environment would be to sanitize the working environment effectively before continue working. It could be food preparation, preparing feeding for dairy calves, chicken houses, bird cages, shelters, restaurants, home cooking and food preparation.

The value could also be the sanitizing of public areas, where hygiene factors such as toilets or urinals or cleaning hard surfaces in shops the public use. The same principles and values and codes reign in households, public schools, offices and other public areas.

Mode of Function of Bacillus bacteria as Bio-Enzymes
In contrast to the SABS standard, being the quickest time a sanitizer (QAC- based) is supposed to kill, Bacillus-containing sanitizers such as the Pierre @ D Bio-Enzymes operate in a different mode. Bio-Enzymes are live bacteria that needs to compete with other microorganisms for a food sources. Hence the longer contact time required by these bacteria to kill its competitors.

1. The pattern that bacillus bacteria uses, (as seen in the study), is to kill or out compete the other bacteria. The answer lies in its morphological structure. Where Bacillus bacteria grows, other microorganisms will move away from that specific waste food source.

2. Bacillus bacteria have a larger morphological structure than other organisms. It creates a zone of inhibition, preventing growth of other microorganisms (known as morphological inhibition).

3. This ensures fewer numbers of other microorganisms surviving, but a rapid growth of bacillus bacteria absorbing waste product such as sewerage, milk spills, waste pills, grease traps etc.

Important knowledge to cfu Calculation
For Bio Enzymes to be most effective and efficient, it is always important to know the cfu value of any biological product that has been diluted. Or in its concentrated form. This is to know if the product you have diluted has sufficient active ingredient to fulfil the desired outcome in waste or stench management:

The ready to use (diluted) product PIERRE @ D BIO ENZYME following thee analyses within SAP – shelf life is typically one year. The cfu count on the diluted product is calculated on a mass balance as follows:

1. Let a = Volume of Bio Enzyme
2. And the concentration = 10 X FF
3. Let a = 1 gallon
4. Let b = volume of water = 9 gallons
5. Let c = volume of Bio Enzyme
6. And a + b = c
7. c = 10 gallons

Doing a mass balance on the spore count:
1. There are 2 x 1012 cfu in 1-gallon Bio Enzyme 10 XFF
2. Count of spores in c = unknown = z Then 2 x 1012a + 0b = zc
3. Then [(2 x 1012 x 1)/10] = [(z x 10)/10]
4. 2 x 1011 cfu/gallon of PIERRE @ D BIO ENZYME

Converting from gallons to liters
2 x 1011 spores 1 gallon
1 gallon 3.8 liters

= 5.2 x 1010 cfu/liter of PIERRE @ D BIO ENZYME
Or 52 billion CFU/liter

The information contained in this leaflet is to the best of our knowledge, true & accurate, but any recommendations or suggestions which may be made are without guarantee since the conditions of use are beyond our control. No license or immunity under any patents is granted or implied.